Anti-Profilin (Tyr-129), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: PFN1
Host: Rabbit
Rrid: AB_3068494
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Profilin (Tyr-129)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Epididymis Li184a Profilin PFN1 PFN2
Clonality: Rabbit Polyclonal
Immunogen: Phospho-Profilin (Tyr-129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr-129 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1. The tyrosine site has some homology to the conserved site (Tyr-129) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to unphosphorylated Profilin (Tyr-129) then affinity purified using Profilin (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 15
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: Profilins are small actin-binding proteins that have functions in cell motility, cytokinesis, gene transcription, endocytosis and neuronal plasticity. Four profilin isoforms have been identified in mammals. Profilin-1 (PFN1) and profilin-2a (PFN2a) isoforms are highly conserved in structure, but PFN1 is ubiquitously expressed while PFN2a is preferentially enriched in brain. In addition, there are two testis-specific profilins, PFN3 and PFN4, that significantly differ in primary sequence and function compared to PFN1 and PFN2a. Profilin is phosphorylated at both tyrosine and serine residues in vivo. Tyr-129 is phosphorylated in response to VEGF-A stimulation, and this promotes profilin actin binding and polymerization. Tyr-129 phosphorylation may be important for angiogenesis induced by injuries. Ser-138 is phosphorylated by ROCK and dephosphorylated by PP1. This serine phosphorylation inhibits G-actin binding, as well as decreases profilin's aggregation suppressor activity by inhibiting binding to huntingtin. Thus, Tyr-129 phosphorylation may activate while Ser-138 phosphorylation may inhibit profilin activity.
Uniprot Number Immunogen Species: P07737
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-129