DESCRIPTION

• Tag: Tag Free
• Accn: NM_001081308
• Type: Mouse Untagged Clone
• Refseq: NM_001081308.2, NP_001074777.1
• Status: 5 Weeks*
• Symbol: Taok3
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 330177
• Synonyms: 2900006A08Rik; A130052D22; A430105I05Rik
• Refseq Orf: 2697 bp
• Uniprot Id: Q8BYC6
• Insert Size: 2697 bp
• Refseq Size: 4258 bp
• Availability: 5 Weeks
• Cytogenetics: 5 F
• Gene Summary: Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses a different segment and lacks an alternate exon in its 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC222498 representing NM_001081308 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGCGTAAAGGGGCACTGAAGGACCCAGAGATTGCTGATCTGTTCTTCAAGGATGACCCCGAAGAGCTTT TTATCGATTTGCATGAAATTGGACATGGGAGCTTTGGAGCCGTGTACTTTGCGACAAATGCGCACACCAA CGAGGTGGTCGCTGTTAAGAAAATGTCCTACAGTGGGAAGCAGACCCATGAGAAATGGCAAGATATCCTT AAGGAAGTTAAGTTTCTGCAGCAGCTGAAGCATCCTAACACCATCGAGTACAAAGGCTGTTATTTAAAGG AGCACACTGCATGGTTGGTGATGGAGTACTGCCTAGGTTCAGCCTCTGACTTGTTAGAAGTTCATAAGAA ACCACTTCAGGAAGTGGAGATCGCTGCTATCACCCATGGTGCCTTGCAGGGGCTGGCCTACCTCCACTTT CACTCCCTGATTCACAGGGACATTAAAGCAGGGAACATCCTCCTCACAGAGCCAGGTCAGGTGAAGCTGG CTGACTTCGGATCTGCCTCCATGGCTTCTCCCGCCAACTCCTTTGTGGGGACTCCTTACTGGATGGCCCC AGAGGTAATCTTAGCTATGGACGAAGGGCAGTACGACGGGAAAGTTGACATCTGGTCGCTTGGCATCACC TGCATAGAGTTGGCGGAGCGGAAGCCCCCCCTCTTCAACATGAACGCCATGAGCGCCCTCTACCACATCG CTCAGAATGACTCCCCCACGCTACAGTCCAGGGAATGGACAGACTCCTTCAGGAGATTCGTTGATTACTG CTTGCACAAAATACCTCAGGAAAGACCAGCCGCGGTGGAGCTGTTGCGGCATGACTTCATTCGGAGGGAG CGGCCACCGAAGGTCCTCATTGATCTCATACAGAGGACAAAAGATGCTGTCCGAGAGCTGGACAACCTGC AGTACAGGAAAATGAAGAAGATCCTCTTCCAGGAGACTCGGAACGGACCCTTGAATGAGTCGCAAGAAGA AGAAGAAGATGGTGAGCAAGGAAGCAACCTGAACCGAGAGGTGGACAGTCTGGGCAGCATCCACTCCATT CCCAGCACATCAGTGAGCACAGGCAGCCGGAGCAGCAGTGTGAACAGCATGCAGGAGGTCATGGATGAGA GCAGCTCTGAGCTCGTCATGATGCAGGAGGATGAAGGCACAGCCAACTCCAGCGCCTCCACGGTACACAA GAAGGATCATGTATTCGTAAGGGATGAGGCGGGCCACGGCGATCCCAGGCCTGAGCCGCGGCCTACCCAG TCAGTTCAGAGCCGGGCCCTCCACTATCGGAACCGAGAGCGCTTTGCCACGATCAAATCCGCGTCTTTGG TTACACGGCAGATCCATGAGCATGAGCAGGAGAACGAGCTGCGGGAACAGATGTCAGGTTATAAGCGGAT GCGGCGCCAGCACCAGAAGCAGCTGATCGCCCTGGAGAACAAGCTGAAGGCTGAGATGGATGAGCACCGC CTCAAGCTCCAGAAGGAGGTGGAGACGCACGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGA AGCAAGTGGCTACCATAGAAAAGGAGGCAAAGGTAGCCGCAGCGGATGAGAAGAAGTTCCAGCAGCAGAT CCTGGCCCAGCAGAAGAAGGACTTGACAACTTTCTTAGAAAGTCAGAAGAAGCAGTACAAGATTTGTAAG GAGAAAATAAAAGAGGAAATGAACGAGGACCATAGCACACCCAAGAAGGAGAAGCAGGAGCGGATATCGA AGCATAAAGAAAACCTGCAGCACACGCAGGCCGAGGAGGAAGCCCACCTGCTCACTCAACAGAGGCTGTA CTACGACAGAAACTGCCGCTTCTTCAAACGGAAAATAATGATCAAGCGCCACGAGGTGGAACAGCAGAAC ATTCGGGAGGAATTAAATAAGAAGAGGACCCAGAAGGAAATGGAGCACGCCATGCTGATCCGGCATGATG AATCCACCCGAGAGCTGGAGTACAGGCAGCTGCACACCCTGCAGAAGCTCCGCATGGATCTGATCCGGCT GCAGCACCAGACGGAACTGGAGAACCAGCTGGAGTACAATAAGAGGCGGGAGCGGGAGCTGCACCGGAAG CACGTCATGGAGCTTCGGCAACAGCCGAAAAACTTAAAGGCCATGGAAATGCAAATTAAAAAACAGTTTC AGGACACTTGCAAAGTACAGACCAAACAGTACAAAGCCCTCAAAAATCACCAGTTGGAAGTCACTCCAAA GAATGAGCACAAAGCAATCCTAAAGACACTGAAGGACGAGCAGACGAGGAAGCTGGCCATTTTGGCCGAG CAGTATGAACAGAGTATAAACGAGATGATGGCCTCTCAAGCGTTACGGCTAGACGAGGCACAAGAGGCAG AGTGCCAGGCCTTGAGACTACAGCTCCAGCAGGAGATGGAGCTGCTCAACGCCTACCAGAGCAAAATCAA GATGCAAACGGAGGCCCAGCACGAGCGCGAGCTACAGAAGCTAGAGCAGAGGGTGTCCCTGCGCAGAGCG CATCTTGAGCAGAAGATTGAAGAGGAGCTGGCTGCCCTGCAGAAGGAACGCAGTGAGAGGATCAAGACTC TCCTGGAGAGACAAGAGCGAGAGACCGAGACTTTTGACATGGAGAGCCTCAGAATGGGCTTTGGGAATTT GGTGACATTAGATTTTCCTAAGGAGGACTATAGATGA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin