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10395-HNAE

Human IL6 / Interleukin-6 Protein

Sino Biological

DETAILS

  • Tag: Native
  • Purity: ≥ 95 % as determined by SDS-PAGE
  • Source: E. coli
  • Species: Human
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: 1. Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc(Cat:10398-H02H), the EC50 of Human IL-6R hFc(Cat:10398-H02H) is 3.0-10.0 ng/mL. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL. 3.Loaded Recombinant Human IL-6R Protein, His Tag (Cat. No. 10398-H08H) on His1K Biosensor, can bind Recombinant Human IL-6 Protein, no Tag (Cat. No. 10395-HNAE) with an affinity constant of 0.165µM as determined in BLI assay (Sartorius Octet RED384)(Routinely tested).
  • Molecule: IL6
  • Endotoxin: < 10 EU per mg of the protein.
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4
  • Molecular Mass: The recombinant human IL6 consists of 184 amino acids and predicts a molecular mass of 20.95 kDa. It migrates as an approximately 19.7 kDa band in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: NP_000591.1
  • Predicted N Terminal: Met

DESCRIPTION

A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.