SARS-CoV-2 Spike Trimer Protein (T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N), His Tag (MALS verified)

DESCRIPTION

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

DETAILS

Tag: C-10×His
Host: HEK293
Size: 50ug
Buffer: PBS
Format: Powder
Purity: 95%
Source: SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ha) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Storage: -20℃
Category: Viral Proteins
Molecule: Spike protein
Accession: QHD43416.1
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Val 16 - Pro 1213
Package Size: 50ug*1
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138 kDa. The protein migrates as 150-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 138 kDa
Shipping Condition: RT
Species Reactivity: SARS-CoV-2
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