EZH2/EED, His-tag, FLAG-tag Recombinant

DESCRIPTION

Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-tag, MW= 87 kDa, and human EED (GenBank Accession No. NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, co-expressed in a baculovirus expression system.

DETAILS

Aa: 2-end (EZH2 and EED)
Mw: 138 kDa (complex)
Tags: N-terminal His-tag (EZH2), N-terminal FLAG-tag (EED)
Format: Aqueous buffer solution
Genbank: NM_004456 (EZH2), NM_003797(EED)
Species: Human
Uniprot: Q15910
Shiptemp: -80°C (dry ice)
Synonyms: KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2; Embryonic Ectoderm Development, hEED, EED, WAIT-1, EZH
Warnings: Avoid freeze/thaw cycles.
Category: Methyltransferase/Enzymes
Background: EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
References: 1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).
Description: Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-tag, MW= 87 kDa, and human EED (GenBank Accession No. NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, co-expressed in a baculovirus expression system.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 200 mM imidazole, and 20% glycerol.
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex. Requires SUZ12 protein for methyltransferase activity.
Host Species: insect
Product Type: Protein
Host Cell Line: Sf9 cells
Biosafety Level: Not applicable (BSL-1)
Storage Stability: At least 6 months at -80°C.
Scientific Category: Methyltransferase