Human IDH1 Protein, His Tag (active enzyme, MALS verified)

DESCRIPTION

The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha‑ketoglutarate，2-HG is elevated in several tumor types, including a subset of AMLs.

DETAILS

Tag: C-10×His
Host: E. coli
Size: 500ug
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
Format: Liquid
Purity: 95%
Source: Human IDH1, His Tag (ID1-H51H6) is expressed from E. coli cells. It contains AA Met 1 - Leu 414 (Accession # O75874-1).
Storage: -70℃
Category: Enzymes
Molecule: IDH1
Accession: NP_005887.2
Conjugate: Unconjugated
Stability: ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
Exp Region: Met 1 - Leu 414
Swiss-prot: O75874-1
Package Size: 500ug*1
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 48.5 kDa
Shipping Condition: Dry ice
Species Reactivity: Human
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