101671-1 101671-2
3CL Protease (T21I, E166V) (SARS-CoV-2) Recombinant
BPS Bioscience
DESCRIPTION
Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct does not contain a tag. The recombinant protein contains the mutations of interests (T21I) and (E166V) and is affinity purified.
DETAILS
- Mw: 34 kDa
- Format: Aqueous buffer solution.
- Purity: ≥90%
- Genbank: YP_009725301
- Species: SARS-CoV-2
- Mutation: T21I, E166V
- Shiptemp: -80°C (dry ice)
- Synonyms: Mpro, Main protease, native 3CL, 3C-like protease, SARS protease, SARS-ncov protease, nsp5
- Warnings: Avoid freeze/thaw cycles
- Category: Coronavirus/Protein
- Constructs: 3CL Protease (T21I, E166V) (1-306(end)) (SARS-CoV-2)
- Description: Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct does not contain a tag. The recombinant protein contains the mutations of interests (T21I) and (E166V) and is affinity purified.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Product Type: Protein
- Host Cell Line: E. coli
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: Assay was run according to 3CL Protease (T21I, E166V) (SARS-CoV-2) Assay Kit (BPS Bioscience #78835) with various amounts of 3CL Protease (T21I, E166V).
- Related Products: 100757, 100739, 79952, 78835
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Coronavirus
- Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.