3CL Protease (T21I, E166V) (SARS-CoV-2) Recombinant

DESCRIPTION

Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct does not contain a tag. The recombinant protein contains the mutations of interests (T21I) and (E166V) and is affinity purified.

DETAILS

Mw: 34 kDa
Format: Aqueous buffer solution.
Purity: ≥90%
Genbank: YP_009725301
Species: SARS-CoV-2
Mutation: T21I, E166V
Shiptemp: -80°C (dry ice)
Synonyms: Mpro, Main protease, native 3CL, 3C-like protease, SARS protease, SARS-ncov protease, nsp5
Warnings: Avoid freeze/thaw cycles
Category: Coronavirus/Protein
Constructs: 3CL Protease (T21I, E166V) (1-306(end)) (SARS-CoV-2)
Description: Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct does not contain a tag. The recombinant protein contains the mutations of interests (T21I) and (E166V) and is affinity purified.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Product Type: Protein
Host Cell Line: E. coli
Biosafety Level: Not applicable (BSL-1)
Assay Conditions: Assay was run according to 3CL Protease (T21I, E166V) (SARS-CoV-2) Assay Kit (BPS Bioscience #78835) with various amounts of 3CL Protease (T21I, E166V).
Related Products: 100757, 100739, 79952, 78835
Storage Stability: At least 6 months at -80°C.
Scientific Category: Coronavirus
Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.