DESCRIPTION

• Tag: Tag Free
• Accn: NM_001252628
• Type: Mouse Untagged Clone
• Refseq: NM_001252628.1, NP_001239557.1
• Status: 3 Weeks*
• Symbol: Cdh16
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 12556
• Refseq Orf: 2448 bp
• Insert Size: 2448 bp
• Refseq Size: 2974 bp
• Availability: 3 Weeks
• Cytogenetics: 8 53.04 cM
• Gene Summary: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 3' UTR and 3' coding region differences, compared to variant 1, which results in a frameshift. The resulting protein (isoform 3) has a distinct C-terminus and is shorter than isoform 1.
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC228987 representing NM_001252628 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGATCTCTGCCCGGCCGTGGCTACTTTACCTCTCTGTTATTCAGGCTTTCACCACTGAGGCCCAGCCTG CAGAAAGCCTGCACACAGAAGTCCCTGAAAACTATGGTGGAAATTTCCCTTTTTACATACTCAAGCTACC ACTACCCCTGGGGAGAGATGAAGGCCACATTGTCCTATCAGGAGACTCAAACACGGCAGATCAAAACACC TTTGCTGTGGACACAGACTCTGGCTTTCTAGTGGCGACAAGGACCCTGGACCGGGAAGAGAAAGCAGAAT ACCAACTACAGGTCACCTTGGAGTCTGAGGATGGACGTATCTTGTGGGGTCCACAGCTTGTGACTGTGCA TGTGAAAGATGAGAATGACCAGGTACCCCAATTCTCCCAGGCCATCTACAGAGCTCAGCTGAGCCAGGGC ACCAGGCCTGGGGTCCCCTTCCTCTTCCTTGAGGCTTCTGATGGGGATGCACCAGGCACAGCTAACTCCG ACCTTCGCTTCCACATTCTGAGCCAGTCCCCACCTCAGCCTTTACCAGACATGTTCCAGCTGGACCCTCA CCTAGGGGCTCTGGCTCTTAGTCCCAGTGGAAGCACCAGCCTAGACCATGCCCTTGAAGAGACTTACCAG CTATTGGTACAGGTCAAGGACATGGGTGACCAGCCTTCAGGCCACCAGGCTATTGCAACTGTAGAGATCT CCATAGTAGAGAACAGCTGGGCACCCCTAGAGCCTGTTCACCTGGCAGAGAATCTCAAAGTTGTGTACCC ACACAGCATTGCCCAGGTGCACTGGAGTGGAGGAGACGTGCACTACCAGCTGGAGAGCCAGCCTCCAGGA CCCTTCGATGTGGATACAGAGGGGATGCTCCATGTTACCATGGAGCTGGACCGGGAGGCCCAGGCTGAGT ACCAGCTCCAAGTCCGAGCTCAGAATTCCCATGGTGAGGACTACGCAGAACCCCTGGAGTTGCAGGTGGT GGTGATGGATGAAAACGACAATGCACCTGTCTGCTCCCCACATGACCCAACAGTCAACATCCCTGAGCTC AGCCCCCCAGGAACTGAAATAGCCAGGCTCTCAGCAGAGGATTTGGATGCCCCTGGGTCACCCAATTCCC ACATTGTATATCAGTTGTTGAGCCCTGAGCCTGAGGAGGGGGCTGAAAACAAAGCCTTCGAGTTAGATCC GACCTCAGGCAGTGTAACACTGGGAACTGCCCCACTCCATGCTGGCCAGAGTATCCTGCTTCAGGTGCTG GCTGTTGACCTAGCAGGATCAGAGAGTGGCCTCAGCAGCACATGTGAGGTGACAGTCATGGTGACAGACG TCAACAACCATGCCCCTGAGTTCATCAATTCCCAGATTGGGCCTGTAACTCTTCCTGAGGATGTAAAACC TGGGGCTCTGGTGGCAACACTCATGGCCACTGATGCTGACCTTGAACCTGCCTTCCGCCTTATGGACTTT GCCATTGAAGAAGGAGACCCAGAAGGGATCTTTGACCTGTCCTGGGAGCCAGACTCCGACCATGTCCAGC TCAGACTCCGGAAGAACCTCAGCTATGAGGCAGCTCCTGATCACAAGGTGGTGGTGGTCGTGAGTAACAT AGAAGAACTGGTGGGCCCAGGCCCAGGCCCTGCAGCCACAGCCACAGTGACTATACTAGTGGAGAGGGTG GTTGCTCCCCTCAAGTTGGACCAGGAGAGCTATGAGACCAGCATCCCAGTCAGCACCCCAGCTGGCTCCC TCCTGCTGACCATCCAGCCCTCAGACCCCATGAGCAGAACCCTCAGGTTCTCCCTGGTCAATGACTCAGA GGGCTGGCTCTGTATCAAGGAGGTGTCTGGGGAGGTACACACAGCCCAGTCCCTGCAGGGTGCCCAGCCT GGAGACACATACACAGTGCTTGTGGAGGCCCAAGACACAGATAAGCCAGGACTGAGCACTTCTGCCACTG TTGTGATCCACTTCCTGAAGGCCTCTCCTGTCCCAGCATTGACTCTGTCTGCTGGGCCCAGCCGACACCT CTGTACACCCCGCCAAGACTACGGTGTAGTTGTGAGTGGGGTCAGTGAGGACCCTGACCTAGCCAACAGG AATGGTCCCTACAGCTTTGCTCTCGGTCCCAATCCCACTGTGCAGCGGGATTGGCGCCTCCAGCCTCTCA ACGATTCCCACGCCTACCTCACCTTGGCATTGCATTGGGTAGAGCCTGGTGAATACATGGTACCTGTGGT TGTCCACCATGATACCCATATGTGGCAACTCCAGGTCAAAGTGATTGTGTGTCGCTGCAACGTGGAAGGC CAATGTATGCGCAAGGTGGGTCGCATGAAGGGAATGCCCACGAAACTGTCAGCGGTGGGTGTCCTCTTGG GCACCCTGGCAGCGATAGGTCACTTGCCCAGATGTTCACATTTAGGAAGCCTGTCTCCACATCCCTAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin