RC223351
PCDHGA11 (NM_032091) Human Tagged ORF Clone
Origene Technologies, Inc.
DESCRIPTION
PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 2
DETAILS
- Mw: 88.6 kDa
- Tag: Myc-DDK
- Accn: NM_032091
- Type: Human Tagged ORF Clone
- Refseq: NM_032091.2
- Status: 4 Weeks*
- Symbol: PCDHGA11
- Vector: pCMV6-Entry
- Category: cDNA Clones
- Locus Id: 56105
- Orf Size: 2511 bp
- Synonyms: PCDH-GAMMA-A11
- Refseq Orf: 2514 bp
- Uniprot Id: Q9Y5H2
- Refseq Size: 3117 bp
- Availability: 4 Weeks
- Cytogenetics: 5q31.3
- Gene Summary: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC223351 representing NM_032091 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCGAATCGGCTACAGCGCGGGGACCGCAGTCGGCTGCTGCTGCTGCTGTGCATTTTCCTGGGGACGC TGCGGGGGTTCCGGGCCAGGCAGATCCGATATTCGGTGCCAGAAGAGACCGAAAAGGGCTCCTTCGTGGG CAATATCTCCAAGGACCTGGGGCTGGAGCCCCGGGAGCTGGCGAAGCGCGGAGTCCGCATCGTCTCCAGA GGGAAGACACAGCTTTTCGCTGTGAATCCGCGAAGCGGCAGCTTGATCACGGCAGGCAGGATAGACCGGG AGGAGCTCTGTGAGACGGTGTCCTCCTGTTTTTTAAATATGGAACTTCTCGTGGAAGACACCTTGAAGAT TTACGGAGTGGAGGTGGAAATAATAGATATTAATGATAACGCCCCCAGCTTCCAGGAGGACGAAGTGGAG ATAAAAGTCAGTGAGCACGCAATTCCTGGGGCGCGATTTGCTCTTCCTAATGCTAGGGATCCAGATGTGG GCGTGAACTCCCTCCAGAGCTACCAGCTCAGCCCTAATAATTACTTTTCCTTGCAACTGCGGGGCAGAAC GGATGGGGCCAAGAATCCAGAGCTAGTACTGGAGGGAAGCCTGGACCGAGAGAAAGAGGCTGCTCACCTG CTCCTCCTCACAGCTTTAGATGGAGGCGATCCCATCCGAAAGGGCGCAGTTCCCATTCGTGTGGTGGTCC TCGATGTAAATGATCACATCCCAATGTTTACACAGTCCGTATATCGCGTGAGTGTTCCAGAAAACATCAG CTCCGGAACTCGGGTGCTGATGGTTAATGCAACGGATCCAGACGAGGGAATCAACGGGGAAGTAATGTAT TCATTTCGGAACATGGAAAGCAAGGCTTCTGAAATATTCCAATTGGATTCACAAACTGGAGAAGTTCAAG TACGGGGGTCTCTGGATTTTGAAAAATATAGATTCTATGAGATGGAAATTCAAGGCCAAGATGGTGGAGG TCTCTTTACCACCACGACGATGTTGATCACTGTTGTGGATGTGAATGATAACGCTCCAGAAATAACTATC ACCTCTTCTATTAATTCAATTCTGGAAAACTCTCCTCCAGGTACAGTGATTGCTCTTCTAAATGTGCAAG ATCAAGATTCTGGAGAAAATGGTCAAGTCTCCTGTTTTATTCCTAACCACCTGCCTTTTAAATTAGAAAA GACTTATGGAAATTATTACAAATTGATAACAAGCAGAGTGCTGGACAGGGAGTTGGTCCAGAGCTACAAT ATAACGTTGACAGCCACAGACCAGGGAAGCCCGCCTTTGTCTGCAGAAACTCATGTCTGGCTGAATGTGG CAGATGACAACGATAACCCTCCCGTTTTTCCTCACTCCTCTTACTCTGCCTACATTCCCGAAAACAACCC CAGGGGTGCCTCCATCTTCTCAGTGACCGCCCTCGACCCGGACAGCAAACAGAATGCCCTGGTCACTTAC TCTCTGACGGATGACACTGTCCAGGGGGTGCCTCTGTCCTCCTATGTCTCTATTAACTCCAACACTGGTG TTCTCTATGCCCTACAATCCTTCGACTATGAGCAGTTTCGAGACTTAGAACTGAGAGTGATAGCACGTGA CAGCGGGGACCCGCCCCTCAGCAGCAACGTGTCGCTGAGCCTGTTCGTGCTGGACCAGAACGACAATGCG CCCGAGATCCTGTACCCTGCCCTCCCCACAGACGGCTCCACTGGCGTGGAGCTGGCGCCCCGCTCTGCGG AACCTGGCTACCTGGTGACCAAGGTGGTTGCGGTGGACAAAGATTCAGGCCAGAACGCCTGGCTGTCCTA TCGCCTGCTTAAGGCCAGCGAGCCGGGACTCTTCGCGGTGGGGGAGCACACGGGCGAGGTGCGTACAGCG CGGGCACTGCTGGACAGAGACGCGCTCAAGCAGAGCCTCGTGGTGGCCGTCCAGGACCACGGCCAGCCCC CTCTCTCGGCCACCGTCACGCTCACCGTGGCTGTGGCCGACAGCATCCCCGAAGTCCTGGCGGACCTCGG CAGCCTCGAGTCTCTGGCTAACTCTGAAACCTCAGACCTCTCGCTGTACTTGGTGGTGGCGGTGGCCGCA GTCTCCTGCATCTTCCTGGTCTTTGTCATCGTGCTGCTGGCACTCAGGCTGTGGCGCTGGCATAAGTCAC GCCTGCTGCAGGCTTCTGAAGGCGGGTTGGCAGGTATGCCCACGTCACATTTTGTAGGCGTGGACGGGGT ACAGGCTTTCCTGCAAACCTATTCCCACGAGGTCTCTCTCATTGCGGACTCGCAGAAGAGTCACCTGATT TTCCCCCAGCCCAACTATGGGGACACGCTCATCAGCCAGGAGAGCTGTGAGAAAAGCGAGCCACTCTTGA TAGCTGAAGACTCAGCTATCATTTTAGGCAAATGTGACCCGACAAGTAATCAGGTGAGATTTATTTCTCT GCCTCCTAATTGTTGGTGTCTTGGCACAAGTCTTTTAAGGAGATGTTTTTTGAGCCTGTTA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC223351 representing NM_032091 Red =Cloning site Green =Tags(s) MANRLQRGDRSRLLLLLCIFLGTLRGFRARQIRYSVPEETEKGSFVGNISKDLGLEPRELAKRGVRIVSR GKTQLFAVNPRSGSLITAGRIDREELCETVSSCFLNMELLVEDTLKIYGVEVEIIDINDNAPSFQEDEVE IKVSEHAIPGARFALPNARDPDVGVNSLQSYQLSPNNYFSLQLRGRTDGAKNPELVLEGSLDREKEAAHL LLLTALDGGDPIRKGAVPIRVVVLDVNDHIPMFTQSVYRVSVPENISSGTRVLMVNATDPDEGINGEVMY SFRNMESKASEIFQLDSQTGEVQVRGSLDFEKYRFYEMEIQGQDGGGLFTTTTMLITVVDVNDNAPEITI TSSINSILENSPPGTVIALLNVQDQDSGENGQVSCFIPNHLPFKLEKTYGNYYKLITSRVLDRELVQSYN ITLTATDQGSPPLSAETHVWLNVADDNDNPPVFPHSSYSAYIPENNPRGASIFSVTALDPDSKQNALVTY SLTDDTVQGVPLSSYVSINSNTGVLYALQSFDYEQFRDLELRVIARDSGDPPLSSNVSLSLFVLDQNDNA PEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDKDSGQNAWLSYRLLKASEPGLFAVGEHTGEVRTA RALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADSIPEVLADLGSLESLANSETSDLSLYLVVAVAA VSCIFLVFVIVLLALRLWRWHKSRLLQASEGGLAGMPTSHFVGVDGVQAFLQTYSHEVSLIADSQKSHLI FPQPNYGDTLISQESCEKSEPLLIAEDSAIILGKCDPTSNQVRFISLPPNCWCLGTSLLRRCFLSLL TR TRPLEQKLISEEDLAANDILDYKDDDDK V
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
- E Coli Selection: Kanamycin (25 ug/mL)
- Protein Families: Transmembrane
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin