DESCRIPTION

• Aa: 224-529
• Mw: 62 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_002834
• Species: Human
• Uniprot: Q06124
• Shiptemp: -80°C (dry ice)
• Synonyms: SHP2, CFC, NS1, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, MGC14433
• Warnings: Avoid freeze/thaw cycles.
• Category: Protein Phosphatase/Non-Receptor
• Background: Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
• References: 1. B.G. Neel et al. Trends Biochem. Sci. (2003) 28:284. 2. J.V. Ravetch and L.L. Lanier Science (2000) 290:84. 3. S. Yamazaki et al. J. Infect. Dis. (2003) 187:334.
• Description: Protein tyrosine phosphatase SHP-2, also known as PTPN11 (GenBank accession number NM_002834), a.a. 224-529 (catalytic domain) with N-terminal GST-tag, MW=62 kDa, expressed in an E. coli expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
• Related Products: 30021, 79018, 79317, 79319-2, 79319-1, 79330
• Specific Activity: >1,000 pmol/min/µg
• Storage Stability: >12 months at -80°C
• Scientific Category: PTP/Non-rec.