DESCRIPTION

• Notes: To generate a Firefly Luciferase-eGFP reporter stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of Geneticin, Hygromycin, or Puromycin (as pre-determined from a killing curve) for antibiotics selection of transduced cells. Visit Cell Line FAQs (bpsbioscience.com) "What is a kill curve?" for more information. Biosafety: The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles
• Category: Cell Signaling Pathway
• Description: The Firefly Luciferase-eGFP Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce firefly luciferase and eGFP (Luc2-P2A-eGFP) driven by an EF1A promoter (Figure 1). The luciferase and eGFP proteins are co-expressed under the EF1A promoter in the cells, allowing greater flexibility for detection of the transduced cells. Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-eGFP Lentivirus (Geneticin). Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-eGFP Lentivirus (Hygromycin). Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase-eGFP Lentivirus (Puromycin).
• Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
• Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: Ideal as a positive control for transduction. Optimize transduction assays and track reporter protein expression over time. Generate a stable cell line expressing Firefly Luciferase and eGFP with Geneticin, Hygromycin, or Puromycin selection.
• Product Type: Lentivirus
• Biosafety Level: BSL-2
• Related Products: 79692, 79980, 78639, 78740
• Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 
• Scientific Category: Cell Signaling Pathway