mouse monoclonal IgG2a; nephrin Antibody (B-12) is an IgG2a κ mouse monoclonal nephrin antibody (also designated nephrin antibody or NPHS1 antibody) that detects nephrin of mouse, rat and human origin by WB, IP, IF and ELISA. nephrin Antibody (B-12) is available as the non-conjugated anti-nephrin antibody form, as well as multiple conjugated forms of anti-nephrin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, eight Ig-like modules, and one fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.