DESCRIPTION

• Mw: 49.3 kDa
• Tag: Myc-DDK
• Accn: NM_019597
• Type: Human Tagged ORF Clone
• Refseq: NM_019597.5
• Status: In Stock*
• Symbol: HNRPH2
• Vector: pCMV6-Entry
• Domains: RRM
• Category: cDNA Clones
• Locus Id: 3188
• Orf Size: 1347 bp
• Synonyms: FTP3; hnRNPH'; HNRPH'; HNRPH2; MRXSB; NRPH2
• Refseq Orf: 1350 bp
• Uniprot Id: P55795
• Refseq Size: 2392 bp
• Availability: In Stock
• Cytogenetics: Xq22.1
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. Read-through transcription between this locus and the ribosomal protein L36a gene has been observed. [provided by RefSeq, Jan 2011]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC221204 ORF sequence Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGATGCTGAGCACGGAAGGCAGGGAGGGGTTCGTGGTGAAGGTCAGGGGCCTACCCTGGTCCTGCTCAG CCGATGAAGTGATGCGCTTCTTCTCTGATTGCAAGATCCAAAATGGCACATCAGGTATTCGTTTCATCTA CACCAGAGAAGGCAGACCAAGTGGTGAAGCATTTGTTGAACTTGAATCTGAAGAGGAAGTGAAATTGGCT TTGAAGAAGGACAGAGAAACCATGGGACACAGATACGTTGAAGTATTCAAGTCTAACAGTGTTGAAATGG ATTGGGTGTTGAAGCATACAGGTCCGAATAGCCCTGATACTGCCAACGATGGCTTCGTCCGGCTTAGAGG ACTCCCATTTGGCTGTAGCAAGGAAGAGATTGTTCAGTTCTTTTCAGGGTTGGAAATTGTGCCAAATGGG ATGACACTGCCAGTGGACTTTCAGGGGCGAAGCACAGGGGAAGCCTTTGTGCAGTTTGCTTCACAGGAGA TAGCTGAGAAGGCCTTAAAGAAACACAAGGAAAGAATAGGGCACAGGTACATTGAGATCTTCAAGAGTAG CCGAGCTGAAGTTCGAACCCACTATGATCCCCCTCGAAAGCTCATGGCTATGCAGCGGCCAGGTCCCTAT GATAGGCCGGGGGCTGGCAGAGGGTATAATAGCATTGGCAGAGGAGCTGGGTTTGAAAGGATGAGGCGTG GTGCCTATGGTGGAGGGTATGGAGGCTATGATGACTATGGTGGCTATAATGATGGATATGGCTTTGGGTC TGATAGATTTGGAAGAGACCTCAATTACTGTTTTTCAGGAATGTCTGATCATAGATACGGAGATGGTGGG TCCAGTTTCCAGAGCACCACAGGGCACTGTGTACACATGAGGGGGTTACCTTACAGAGCCACTGAGAATG ATATTTATAATTTCTTCTCACCTCTTAATCCCATGAGAGTACATATTGAAATTGGACCCGATGGCAGAGT TACCGGTGAGGCAGATGTTGAATTTGCTACTCATGAAGATGCTGTGGCAGCTATGGCAAAAGACAAAGCT AATATGCAACACAGATATGTGGAGCTCTTCTTAAATTCTACTGCAGGAACAAGTGGGGGTGCTTACGATC ACAGCTATGTAGAACTTTTTTTGAATTCTACAGCAGGGGCAAGTGGTGGCGCTTATGGTAGCCAAATGAT GGGAGGGATGGGCTTATCCAACCAGTCTAGTTATGGAGGTCCTGCTAGCCAGCAGCTGAGTGGTGGTTAT GGAGGTGGTTATGGTGGTCAGAGCAGTATGAGTGGATATGACCAAGTTCTGCAGGAAAACTCCAGTGACT ATCAGTCAAACCTTGCT ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC221204 protein sequence Red =Cloning site Green =Tags(s) MMLSTEGREGFVVKVRGLPWSCSADEVMRFFSDCKIQNGTSGIRFIYTREGRPSGEAFVELESEEEVKLA LKKDRETMGHRYVEVFKSNSVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNG MTLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPY DRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYGGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGG SSFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPMRVHIEIGPDGRVTGEADVEFATHEDAVAAMAKDKA NMQHRYVELFLNSTAGTSGGAYDHSYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGY GGGYGGQSSMSGYDQVLQENSSDYQSNLA TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin