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SPN-C52Hp-50ug SPN-C52Hp-500ug

SARS-CoV-2 Spike Trimer Protein (N501Y, D614G), His Tag (MALS verified)

ACROBiosystems

DESCRIPTION

SARS-CoV-2 S protein (N501Y,D614G), His Tag (SPN-C52Hp) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (N501Y, D614G)). The N501Y/D614G mutations were identified in multiple SARS-CoV-2 variants which emerged in the United Kingdom (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01), South Africa(known as B.1.351 or 20C/501Y.V2), and Brazil (Known as P.1). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

DETAILS

  • Tag: C-10×His
  • Host: HEK293
  • Size: 50ug
  • Buffer: PBS
  • Format: Powder
  • Purity: 95%
  • Storage: -20℃
  • Category: Viral Proteins
  • Molecule: Spike protein
  • Accession: QHD43416.1 (N501Y, D614G)
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Exp Region: Val 16 - Pro 1213
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 170-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 138.0 kDa
  • Shipping Condition: RT
  • Species Reactivity: SARS-CoV-2
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html