11089-K08H
Rhesus DKK-1 / Dkk1 Protein (256 Asn/Gln, His Tag)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the rhesus DKK1 (XP_001098844.1) precursor (Met 1-His 266) (256 Asn / Gln) was expressed, fused with a C-terminal polyhistidine tag.
DETAILS
- Tag: C-His
- Purity: > 95 % as determined by SDS-PAGE
- Source: HEK293 Cells
- Species: Rhesus
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells . The ED50 for this effect is typically 0.5-3 μg/ml in the presence of 10 ng/mL of Wnt3a.
- Molecule: DKK1
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4
- Molecular Mass: The recombinant rhesus DKK1 comprises 246 amino acids with a predicted molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhesus DKK1 is approximately 38-43 kDa in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: XP_001098844.1
- Predicted N Terminal: Thr 32