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APC-047_25 mcl APC-047_50 mcl APC-047_0.2 ml APC-047-CF_0.2 ml

Scientist.com Supplier

Anti-KCNK2 (TREK-1) Antibody

Alomone Labs

DESCRIPTION

Anti-KCNK2 (TREK-1) Antibody (#APC-047) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TREK-1 from mouse, rat, and human samples.

DETAILS

  • Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
  • Host: Rabbit
  • Clone: NA
  • Label: Unconjugated
  • Purity: Affinity purified on immobilized antigen.
  • Target: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 3776
  • Isotype: Rabbit IgG
  • Homology: Rat - identical; mouse - 17/18 amino acid residues identical
  • Is Toxin: No
  • Sequence: DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 
  • Synonyms: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
  • Accession: O95069
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Human|Rat|Mouse
  • Formulation: PBS pH7.4
  • Applications: IC|IF|IHC|WB
  • Ko-validated: yes
  • Preservative: No Preservative
  • Reconstitution: 0.2 ml double distilled water (DDW).
  • Blocking Peptide: BLP-PC047
  • Negative Control: BLP-PC047
  • Positive Control: NA
  • Cited Application: IP|IHC|ICC
  • Immunogen Location: Intracellular, N-terminus
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Human
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: IC|IF|IHC|WB
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: KCNK2 (also named TWIK-related K+ channel, TREK-1 or K2P2.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK2 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, KCNK2 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.KCNK2 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while KCNK2 expression in rodents is more widespread.KCNK2 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that KCNK2 may be a potential target for anti-depressants.KCNK2 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gαi, dissociation of Gβγ, followed by the opening of the glutamate permeable KCNK2 (TREK-1) K+ channel through its interaction with Gβγ. For this purpose, KCNK2 is localized at the cell surface of the cell body and processes of astrocytes.
  • Standard Quality Control of Each Lot: Western blot analysis
  • Application Dilutions Western Blot Wb: 1:200
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information