DESCRIPTION

• Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
• Host: Rabbit
• Clone: NA
• Label: Unconjugated
• Purity: Affinity purified on immobilized antigen.
• Target: FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 25449
• Isotype: Rabbit IgG
• Homology: Mouse, human - 12/13 amino acid residues identical
• Is Toxin: No
• Sequence: (C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSHR
• Synonyms: FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO
• Accession: P20395
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Human|Mouse
• Formulation: PBS pH7.4
• Applications: IC|IF|IHC|LCI|WB
• Preservative: No Preservative
• Reconstitution: 0.2 ml double distilled water (DDW).
• Blocking Peptide: BLP-FR010
• Negative Control: BLP-FR010
• Positive Control: NA
• Immunogen Location: Extracellular, N-terminus
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Rat
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: IC|IF|IHC|LCI|WB
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: FSH (follicle stimulating hormone) is the central hormone of mammalian reproduction, necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life1. Together with LH, this gonadotropin is produced and secreted by the pituitary gland as a highly heterogeneous glycoprotein2. The FSH receptor belongs to the family of G-protein coupled receptors, complex transmembrane proteins characterized by seven hydrophobic helices inserted in the plasma membrane and by intracellular and extracellular domains of variable dimensions depending on the type of ligand3. The intracellular portion of the FSH receptor is coupled to a Gs protein and, upon receptor activation by the hormonal interaction with the extracellular domain it initiates a cascade of events that ultimately leads to the specific biological effects of the gonadotropin.FSH acts by binding to specific receptors, localized exclusively in the gonads. The FSH receptor is expressed by two cell types of the gonad, namely Sertoli cells in the testis, and granulosa cells in the ovarian follicle4.Mutations of FSHR may cause primary or secondary amenorrhea, infertility, premature ovarian failure (POF) and ovarian hyperstimulation syndrome (OHSS)5. Mutations of FSHR may also play a role in the development of granulosa cell tumors of ovaries6.
• Standard Quality Control of Each Lot: Western blot analysis
• Application Dilutions Western Blot Wb: 1:200
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:200