DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Shiptemp: -80°C (dry ice)
• Synonyms: Groundhog, Marmota Monax, PD-1, Programmed Cell Death 1, PDCD1, SLEB2, CD279, NFAT
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Cell Line
• Background: The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated Tcells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.
• Description: Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of woodchuck (groundhog, Marmota monax) PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #HQ403652).
• Supplied As: Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: • Screen for activators or inhibitors of woodchuck PD-1 signaling in a cellular context • Characterize the biological activity of woodchuck PD-1 and its interactions with ligands
• Host Species: human
• Product Type: Cell Line
• Host Cell Line: Jurkat
• Biosafety Level: BSL-1
• Related Products: 60690-1, 79457, 79455, 60690, 60184, 79796
• Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
• Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
• Scientific Category: Immunotherapy
• Instructions for Use: See data sheet for detailed culturing and assay protocol.
• Required Accessories: 60690,60184,79796
• Signed License Required: Yes