DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: Protein Arginine Methyltransferase 7, [Myelin Basic Protein]-Arginine N-Methyltransferase PRMT, Histone-Arginine N-Methyltransferase PRMT7, Protein Arginine N-Methyltransferase 7, EC 2.1.1.321, KIAA1933, EC 2.1.1, SBIDDS
• Warnings: Avoid multiple freeze/thaw cycles.
• Category: Methyltransferase/Assay Kit
• References: 1. You Feng, et al. 2014. J Biol Chem 289:32604.2. Pengyu Geng, et al. 2017. FASEB J. 31:2287.
• Description: The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial-mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Note: To study methylation of histone proteins by PRMT7, we recommend using our PRMT7 Homogeneous Assay Kit (H2b), #79720.
• Methodology: AlphaLISA®
• Supplied As: The PRMT7 Homogeneous Assay Kit (Automethylation) comes in a convenient AlphaLISA® format, with S-adenosylmethionine (SAM), primary antibody, methylation assay buffer, and purified Flag-tagged PRMT7 for 384 enzyme reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Great for studying enzyme kinetics and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 52055, 52054, 51054, 79720
• Contraindications: Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.
• Storage Stability: At least one year from date of receipt when stored as directed.
• Scientific Category: Methyltransferase
• Instructions for Use: See assay protocol for detailed instructions.