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ANX-002_25 mcl ANX-002_50 mcl ANX-002_0.2 ml ANX-002-CF_0.2 ml

Scientist.com Supplier

Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody

Alomone Labs

DESCRIPTION

Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE-2 from human, rat, and mouse samples.

DETAILS

  • Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
  • Host: Rabbit
  • Clone: NA
  • Label: Unconjugated
  • Purity: Affinity purified on immobilized antigen.
  • Target: Sodium/hydrogen exchanger 2, SLC9A2
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 24783
  • Isotype: Rabbit IgG
  • Homology: Mouse - identical; human - 12/16 amino acid residues identical
  • Is Toxin: No
  • Sequence: (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2
  • Synonyms: Sodium/hydrogen exchanger 2, SLC9A2
  • Accession: P48763
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Human|Rat|Mouse
  • Formulation: PBS pH7.4
  • Applications: IF|IHC|WB
  • Preservative: No Preservative
  • Reconstitution: 0.2 ml double distilled water (DDW).
  • Blocking Peptide: BLP-NX002
  • Negative Control: BLP-NX002
  • Positive Control: NA
  • Immunogen Location: Intracellular, C-terminus
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Rat
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: IF|IHC|WB
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8.NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12.
  • Standard Quality Control of Each Lot: Western blot analysis
  • Application Dilutions Western Blot Wb: 1:200
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:50