CHK2 Kinase Assay Kit

DESCRIPTION

The CHK2 Kinase Assay Kit is designed to measure CHK2 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.

DETAILS

Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
Species: Human
Uniprot: O96017
Shiptemp: -80°C (dry ice)
Synonyms: Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2, CDS1, CHK2, RAD53
Warnings: Avoid freeze/thaw cycles.
Category: Kinase/Assay Kit
Background: CHK2 (checkpoint kinase 2, also known as CHEK2) is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint in response to DNA damage. Thus, it is rapidly phosphorylated and activated upon DNA damage. It is known to phosphorylate and inactivate phosphatase Cdc25C, thereby preventing entry into mitosis. CHK2 also stabilizes tumor suppressor TP53 and is a key player in the ATM DNA damage response and repair pathway. CHK2 mutations are observed in Li-Fraumeni syndrome, an inherited predisposition to cancer. Mutations in CHK2 are also linked to various spontaneous types of cancer.
Description: The CHK2 Kinase Assay Kit is designed to measure CHK2 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Methodology: Chemiluminescent
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant CHK2 kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Unspsc Code: 41105332
Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Related Products: 40039, 40040, 78418, 78487
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Scientific Category: Kinase