DESCRIPTION

• Aa: 2-634(end)
• Mw: 98 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_005020
• Species: Human
• Uniprot: Q14123
• Shiptemp: -80°C (dry ice)
• Synonyms: PDE1C, phosphodiesterase 1C
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Vandeput, F., et al. (2007). J. Biol. Chem. Nov 9;282(45):32749-57. 2. Cygnar, K.D., Zhao, H. (2009). Nat. Neurosci. Apr;12(4):454-62.3. Ortiz-Capisano, M.C., et al. (2009). Am. J. Physiol. Regul. Integr. Comp. Physiol. Nov;297(5):R1469-76. Application Reference(s): A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
• Description: Human PDE1C (GenBank Accession No. NM_005020), a.a. 2-634(end), with N-terminal GST-tag, MW=98 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20, and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
• Assay Conditions: Assayed using BPS's PDE1C Assay Kit, Cat. # 60312. Assay conditions: 10 mM Tris-HCl,_x000D_ pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,_x000D_ 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and_x000D_ serial dilutions of PDE1C at 37°C for 20 min._x000D_ Quantified by 5'- nucleotidase cleaving the_x000D_ 5' -AMP product and releasing the_x000D_ phosphate group which is detected by_x000D_ Malachite Green Reagent.
• Related Products: 61312, 60705, 60312, 60012
• Specific Activity: ≥ 15,000 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phosphodiesterase