36-1628
Monoclonal Antibody to Bax (Apoptosis Marker)(Clone : SPM336)
Abeomics
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DESCRIPTION
Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
DETAILS
- Gene: BAX
- App Id: FACS,IF,WB,IHC
- Antigen: Bax (Apoptosis Marker)
- Gene Id: 581
- Isotype: Mouse IgG1, kappa
- Format Id: Purified
- Clone Name: SPM336
- Uniprot Id: Q07812
- Host Animal: Mouse
- Clonality Id: Monoclonal
- Purification: Affinity Chromatography
- Reactivity Id: Human
- Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
- Alternative Name: BAX||BCL2L4
- Application Note: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes),
- Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
- Immunogen Information: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.