DESCRIPTION

• Aa: 1-979 (end), delta 346-731
• Mw: 92 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_033116
• Species: Human
• Uniprot: Q8TD19
• Shiptemp: -80°C (dry ice)
• Synonyms: NEK9, NIMA-related kinase 9, Never In Mitosis-Related Kinase 9, NERCC
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• References: 1. Roig, J., et al. Genes Dev. 16: 1640-1658 (2002).2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).
• Description: Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a 1-345, 732-end (a.a. 1-979 with deleted fragment a.a. 346-731), with N-terminal GST-tag, MW =92 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml MBP for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
• Related Products: 40141, 40140, 40136, 40041, 78487
• Specific Activity: 147 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine