40142
NEK9, GST-tag Recombinant
BPS Bioscience
DESCRIPTION
Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a 1-345, 732-end (a.a. 1-979 with deleted fragment a.a. 346-731), with N-terminal GST-tag, MW =92 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
DETAILS
- Aa: 1-979 (end), delta 346-731
- Mw: 92 kDa
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: NM_033116
- Species: Human
- Uniprot: Q8TD19
- Shiptemp: -80°C (dry ice)
- Synonyms: NEK9, NIMA-related kinase 9, Never In Mitosis-Related Kinase 9, NERCC
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase-Serine/Threonine/Protein
- References: 1. Roig, J., et al. Genes Dev. 16: 1640-1658 (2002).2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).
- Description: Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a 1-345, 732-end (a.a. 1-979 with deleted fragment a.a. 346-731), with N-terminal GST-tag, MW =92 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
- Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml MBP for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
- Related Products: 40141, 40140, 40136, 40041, 78487
- Specific Activity: 147 pmol/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Kinase-Serine/Threonine