DESCRIPTION

• Uniprot: Q9NXA8
• Shiptemp: -80°C (dry ice)
• Synonyms: sirt5, sirtuin 5
• Warnings: Avoid freeze/thaw cycles.
• Category: Deacetylase/Assay Kit
• References: 1.J. Du et al. (2011) Science. 334: 806. 2. A. Madsen et al. (2012) J. Med. Chem 55: 5582. 3. F. Fisher et al. (2012) Plos One. 7: 1.
• Description: The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
• Methodology: Fluorogenic
• Supplied As: The Fluorogenic SIRT5 Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Deacetylase
• Instructions for Use: See assay kit datasheet for detailed protocol.