51027
EZH2 (del153Y)/EED/SUZ12/RbAp48/AEBP2, FLAG, His-tag Recombinant
BPS Bioscience
DESCRIPTION
Mutant version of BPS's regular EZH2 5-member complex (Cat. # 51004), but with a Tyr deletion on a.a. 153 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end; del153Y) with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
DETAILS
- Aa: 2-end (all components)
- Mw: 325 kDa (complex)
- Tags: N-terminal FLAG (EED), N-terminal His-tag (rest)
- Format: Aqueous buffer solution
- Genbank: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
- Species: Human
- Uniprot: Q15910
- Shiptemp: -80°C (dry ice)
- Synonyms: KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), hEED, Suppressor Of Zeste 12 Homolog, CHET9, JJAZ1; AE binding protein 2, Adipocyte Enhancer binding protein 2, EZH
- Warnings: Avoid freeze/thaw cycles.
- Category: Methyltransferase/Enzymes
- Background: EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
- References: 1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).2. Rakotobe, D. et al., Virol. J. 5, 32 (2008). Application Reference: Morin, R.D., et al. Nat Genet. 2010 February, 42(2): 181-185.
- Description: Mutant version of BPS's regular EZH2 5-member complex (Cat. # 51004), but with a Tyr deletion on a.a. 153 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end; del153Y) with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
- Supplied As: aqueous buffer solution
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
- Host Species: insect
- Product Type: Proteins
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 51029, 51033, 51032
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Methyltransferase