CO5-H52Hx-100ug CO5-H52Hx-1mg
Human Complement C5 (R885H) Protein, His Tag
ACROBiosystems
DESCRIPTION
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
DETAILS
- Tag: C-10×His
- Host: HEK293
- Size: 100ug
- Buffer: PBS, pH7.4
- Format: Powder
- Purity: 90%
- Source: Human Complement C5 (R885H), His Tag (CO5-H52Hx) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1 (R885H)).
- Storage: -20℃
- Category: Cytokines&Growth Factors
- Molecule: Complement C5
- Accession: NP_001726.2
- Conjugate: Unconjugated
- Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Exp Region: Gln 19 - Cys 1676
- Swiss-prot: P01031-1 (R885H)
- Package Size: 100ug*1
- Characteristics: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.7 kDa (α chain). The protein migrates as 70 kDa (β chain) and 110-120 kDa (α chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 180 kDa under non-reducing (NR) condition (SDS-PAGE).
- Endotoxin Level: 1.0 EU per μg
- Molecular Weight: 73.9 kDa (β chain) & 114.7 kDa (α chain)
- Shipping Condition: RT
- Species Reactivity: Human
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