DESCRIPTION

• Form: Affinity Purified
• Gene: TARDBP
• Host: Rabbit
• Rrid: AB_2492258
• Type: Primary Antibody
• Buffer: PBS + 30% glycerol
• Target: TDP43
• Isotype: IgG
• Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
• Shipping: Blue Ice
• Synonyms: ALS10 antibody, OTTHUMP00000002171 antibody, OTTHUMP00000002172 antibody, OTTHUMP00000002173 antibody, TADBP_HUMAN antibody, TAR DNA binding protein 43 antibody, TAR DNA binding protein antibody, TAR DNA-binding protein 43 antibody, TARDBP antibody, TDP 43 antibody, TDP-43 antibody, TDP43 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of human TDP-43.
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~43 kDa TDP-43 protein in Western blots of rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43.
• Applications: WB, IHC, ICC
• Ncbi Gene Id: 23435
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 43
• Production Notes: Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
• Dilution Range Wb: 1:1000
• Immunogen Species: Human
• Dilution Range Icc: 1:1000
• Dilution Range Ihc: 1:250-1:1000
• Species Reactivity: Human and Rat
• Target Description: TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: Q13148

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