Anti-CD14 (Monocyte / Macrophage Marker) Monoclonal Antibody(Clone: LPSR/2408)

DESCRIPTION

Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

DETAILS

Gene: CD14
App Id: WB,IHC
Gene Id: 929
Isotype: Mouse IgG1, kappa
Clone Name: LPSR/2408
Uniprot Id: P08571
Host Animal: Mouse
Clonality Id: Monoclonal
Reactivity Id: Human
Product Content: 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Alternative Name: Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein
Application Note: Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95&degC followed by cooling at RT for 20 minutes),
Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Immunogen Information: Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)