ViraTrapTMlentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column. Viruses are first applied to a purification column and then further purified and concentrated through a concentration unit. Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once. Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C Read the protocol and instruction manual here: The following materials are required for operation but not included in the kit: Centrifuge for tissue culture work with swing out rotor 0.45um filter unit or syringe filter Rack holder for column PBS Each Lentivirus purification kit includes: (2 Prep, 4 Prep, 20 Prep) LV columns: 1, 2, 5 Press-on cap: 1, 4, 10 Centrifugal filter: 1, 4, 10 50mL conical tube: 2, 8, 20 Buffer P: 10mL, 80mL, 180mL Buffer LS: 5mL, 80mL, 180mL Regeneration buffer: 5mL, 20mL, 40mL