10797-H49H-B
Human CD48 Protein (His & AVI Tag), Biotinylated
Sino Biological
DESCRIPTION
A DNA sequence encoding the human CD48 (NP_001769.2) (Met1-Ser220) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
DETAILS
- Tag: C-His & AVI
- Purity: > 95 % as determined by SDS-PAGE.
- Source: HEK293 Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Immobilized Human 2B4/CD244 (hFc Tag)(Cat:10042-H02H) at 2 μg/mL (100 μL/well) can bind Human CD48 (His & AVI Tag), Biotinylated(Cat:10797-H49H-B), the EC50 is 120-520 ng/mL.
- Molecule: CD48
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4
- Molecular Mass: The recombinant human CD48 consists of 220 amino acids and predicts a molecular mass of 25.60 kDa. It migrates as an approximately 42.34 kDa band in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_001769.2
- Predicted N Terminal: Gln27