DESCRIPTION

• Aa: 2-605(end)
• Mw: 68 kDa
• Tags: N-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_004205
• Species: Human
• Uniprot: O75604
• Shiptemp: -80°C (dry ice)
• Synonyms: USP 2, Ubiquitin-Specific Protease 2, UPB41, USP-2
• Warnings: Avoid freeze/thaw cycles.
• Category: Ubiquitination/Deubiquitylating Enzymes
• References: 1. Stevenson, L.F., et al. (2010). EMBO J. 140(3): 384-96.2. Priolo, C., et al. (2006). Cancer Res. 66(17): 8625-32.3. Haimerl, F., et al. (2009). J. Biol. Chem. 284(1): 495-504.
• Description: Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 2-605(end) with an N-terminal FLAG-tag, MW=68 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl Flag peptide, 20% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation =340 nm, emission =460 nm.
• Related Products: 80358, 80392, 81102, 81103, 81100
• Specific Activity: 8.9 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitins: Deubiquitinase (DUB)