SP1401
Anti-Semaphorin-3A (N-terminal) Antibody
PhosphoSolutions
DETAILS
- Form: Antigen Affinity Purified
- Gene: SEMA3A
- Host: Rabbit
- Rrid: AB_2185389
- Type: Primary Antibody
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: Semaphorin-3A (N-terminal)
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: Sema3A, Semaphorin III
- Clonality: Rabbit Polyclonal
- Immunogen: Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human Sema3A. The sequence used is identical to similar regions in rat and mouse Sema3A, and has low homology to other semaphorin family members.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera. This antibody has not been shown to be reactive in neonatal rat brain, but has weak reactivity in adult mouse brain.
- Applications: WB, ICC
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 95
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Dilution Range Icc: 1:400
- Species Reactivity: Human, Mouse, and Rat
- Target Description: One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
- Uniprot Number Immunogen Species: Q14563