2-1000-025
10xBufferE Strep-Tactin Elution Buffer
IBA Lifesciences
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DESCRIPTION
10x Buffer E contains desthiobiotin and is a 10x concentrated elution buffer for Strep-Tactin® resins. Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the biotin binding pocket of Strep-Tactin®. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins.
DETAILS
- Ph: 8.0
- Category: Buffers & Biochemicals
- Hazardous: No
- Lead Time: 3-5 days
- Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins
- Composition: 1 M Tris-Cl, 1.5 M NaCl 10 mM EDTA, 25 mM desthiobiotin, pH 8.0