DESCRIPTION

• Mw: 76.8 kDa
• Tag: Myc-DDK
• Accn: NM_003500
• Type: Human Tagged ORF Clone
• Refseq: NM_003500.4
• Status: In Stock*
• Symbol: ACOX2
• Vector: pCMV6-Entry
• Domains: ACOX, Acyl-CoA_dh
• Category: cDNA Clones
• Locus Id: 8309
• Orf Size: 2043 bp
• Synonyms: BCOX; BRCACOX; BRCOX; CBAS6; THCCox
• Refseq Orf: 2046 bp
• Uniprot Id: Q99424
• Refseq Size: 2356 bp
• Availability: In Stock
• Cytogenetics: 3p14.3
• Gene Summary: The product of this gene belongs to the acyl-CoA oxidase family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe cognitive disability, and death in children. [provided by RefSeq, Mar 2009]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC208278 ORF sequence Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGGCAGCCCAGTGCACCGAGTGTCATTGGGGGATACCTGGAGCAGGCAAATGCACCCCGACATAGAGA GCGAGAGGTATATGCAGTCCTTTGACGTGGAACGGCTCACCAACATCCTTGATGGAGGTGCCCAGAACAC TGCACTCCGCAGGAAAGTTGAGAGCATCATCCACAGTTACCCGGAGTTTAGCTGTAAGGACAATTATTTC ATGACCCAGAATGAGCGTTATAAGGCTGCCATGCGGAGGGCATTCCACATCCGGTTGATAGCTCGGCGCC TGGGTTGGTTAGAAGATGGTCGTGAATTAGGCTACGCTTACAGAGCCCTTTCTGGAGACGTGGCCTTAAA TATACACAGAGTCTTCGTGAGAGCCCTCAGGAGCCTGGGCTCAGAGGAGCAGATTGCCAAATGGGACCCA CTCTGCAAAAACATCCAGATCATCGCAACGTATGCACAGACAGAGTTGGGACATGGGACATATCTTCAGG GCCTGGAGACTGAAGCCACCTATGACGCAGCCACCCAGGAGTTTGTGATACACAGCCCCACGCTGACTGC CACCAAATGGTGGCCTGGAGACTTGGGACGGTCAGCCACCCATGCCCTGGTCCAGGCCCAGCTGATCTGC TCAGGAGCCAGGCGGGGCATGCACGCTTTTATTGTGCCAATCCGGAGTCTTCAGGACCACACCCCACTGC CAGGAATCATCATTGGGGACATCGGACCCAAGATGGACTTTGATCAAACAGACAATGGCTTCCTGCAGCT GAACCATGTGCGGGTCCCCAGGGAGAACATGCTGAGTCGCTTTGCACAGGTCTTGCCAGATGGCACCTAC GTCAAACTCGGTACAGCACAGAGCAACTACCTTCCCATGGTGGTGGTGCGGGTGGAGCTGCTGTCAGGGG AGATCCTCCCTATACTGCAGAAGGCCTGTGTCATCGCCATGCGCTACTCGGTCATCCGCCGCCAATCCCG GCTCCGGCCCAGTGACCCAGAGGCAAAGGTCCTGGACTACCAGACACAACAGCAGAAACTCTTTCCTCAG CTGGCCATCAGTTATGCCTTCCATTTCCTGGCAGTCAGCCTCTTGGAGTTCTTCCAGCACTCCTACACTG CCATTCTGAACCAAGACTTCAGCTTCCTGCCTGAGCTCCACGCACTGAGCACGGGCATGAAGGCCATGAT GTCAGAATTCTGCACCCAGGGAGCTGAGATGTGCCGCAGGGCCTGTGGCGGACATGGCTACTCAAAGCTG AGTGGCCTGCCATCACTGGTCACCAAATTGTCGGCCTCCTGTACCTACGAGGGTGAGAACACAGTGCTCT ACCTGCAGGTGGCCAGGTTCCTGGTGAAGAGCTACCTGCAGACTCAGATGTCCCCTGGCTCCACGCCACA GAGATCTCTCTCTCCATCTGTCGCATATCTCACCGCACCTGACCTGGCCAGGTGTCCAGCCCAGAGGGCA GCCGACTTCCTCTGCCCGGAGCTCTACACCACGGCCTGGGCACATGTGGCAGTAAGGCTCATAAAGGACT CAGTGCAGCATTTACAGACCCTGACGCAATCCGGAGCTGACCAGCACGAGGCTTGGAACCAGACCACTGT CATACACCTCCAGGCTGCTAAGGTGCACTGCTACTATGTCACTGTGAAGGGTTTTACAGAAGCTCTGGAG AAACTAGAAAATGAACCAGCGATTCAGCAGGTGCTCAAGCGCCTCTGTGACCTCCATGCCATACATGGAA TCTTGACTAACTCGGGTGACTTTCTCCATGACGCCTTCCTGTCTGGTGCCCAAGTGGACATGGCAAGAAC AGCCTACCTGGACCTGCTCCGCCTGATCCGGAAGGATGCCATCCTGTTAACTGATGCTTTTGACTTCACC GATCAGTGTTTAAATTCAGCACTTGGCTGTTATGATGGAAACGTCTACGAACGCCTGTTCCAGTGGGCTC AGAAGTCACCAACCAATACTCAGGAGAACCCTGCCTATGAGGAATATATAAGACCACTTTTACAAAGTTG GAGATCCAAGCTA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC208278 protein sequence Red =Cloning site Green =Tags(s) MGSPVHRVSLGDTWSRQMHPDIESERYMQSFDVERLTNILDGGAQNTALRRKVESIIHSYPEFSCKDNYF MTQNERYKAAMRRAFHIRLIARRLGWLEDGRELGYAYRALSGDVALNIHRVFVRALRSLGSEEQIAKWDP LCKNIQIIATYAQTELGHGTYLQGLETEATYDAATQEFVIHSPTLTATKWWPGDLGRSATHALVQAQLIC SGARRGMHAFIVPIRSLQDHTPLPGIIIGDIGPKMDFDQTDNGFLQLNHVRVPRENMLSRFAQVLPDGTY VKLGTAQSNYLPMVVVRVELLSGEILPILQKACVIAMRYSVIRRQSRLRPSDPEAKVLDYQTQQQKLFPQ LAISYAFHFLAVSLLEFFQHSYTAILNQDFSFLPELHALSTGMKAMMSEFCTQGAEMCRRACGGHGYSKL SGLPSLVTKLSASCTYEGENTVLYLQVARFLVKSYLQTQMSPGSTPQRSLSPSVAYLTAPDLARCPAQRA ADFLCPELYTTAWAHVAVRLIKDSVQHLQTLTQSGADQHEAWNQTTVIHLQAAKVHCYYVTVKGFTEALE KLENEPAIQQVLKRLCDLHAIHGILTNSGDFLHDAFLSGAQVDMARTAYLDLLRLIRKDAILLTDAFDFT DQCLNSALGCYDGNVYERLFQWAQKSPTNTQENPAYEEYIRPLLQSWRSKL TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin