SEKA002
Influenza A H5N1 (Avian Flu) Hemagglutinin / HA ELISA Pair Set
Sino Biological
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DESCRIPTION
The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of H5N1 (avian flu) hemagglutinin.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H5N1 (avian flu) hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any H5N1 (avian flu) hemagglutinin present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-H5N1 (avian flu) hemagglutinin monoclonal antibody is then added, producing an antibody-antigen-antibody ""sandwich"". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H5N1 (avian flu) hemagglutinin present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 300 ng of recombinant H5N1 hemagglutinin. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 125-8000
- Specificity: HA
- Capture Antibody: 1 mg/mL of mouse anti-H5N1 hemagglutinin monoclonal antibody. Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11048-MM14)
- Detection Antibody: 0.2 mg/mL of rabbit anti-Influenza A H5N1 hemagglutinin polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use.