80393
A20, His-tag (E. coli-derived) Recombinant
BPS Bioscience
DESCRIPTION
Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-357 with an N-terminal His-tag, MW=42.8 kDa, expressed in an E. coli cell expression system.
DETAILS
- Aa: 2-357
- Mw: 42.8 kDa
- Tags: N-terminal His-tag
- Format: Aqueous buffer solution
- Genbank: NM_006290
- Species: Human
- Uniprot: P21580
- Shiptemp: -80°C (dry ice)
- Synonyms: A20, tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C
- Warnings: Avoid freeze/thaw cycles.
- Category: Ubiquitination/Deubiquitylating Enzymes
- References: 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
- Description: Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-357 with an N-terminal His-tag, MW=42.8 kDa, expressed in an E. coli cell expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Product Type: Protein
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and A20. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, and emission = 460 nm.
- Specific Activity: 10.5 pmol/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Ubiquitins: Deubiquitinase (DUB)