DESCRIPTION

• Aa: 2-357
• Mw: 42.8 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_006290
• Species: Human
• Uniprot: P21580
• Shiptemp: -80°C (dry ice)
• Synonyms: A20, tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C
• Warnings: Avoid freeze/thaw cycles.
• Category: Ubiquitination/Deubiquitylating Enzymes
• References: 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
• Description: Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-357 with an N-terminal His-tag, MW=42.8 kDa, expressed in an E. coli cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and A20. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, and emission = 460 nm.
• Specific Activity: 10.5 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitins: Deubiquitinase (DUB)