78810-1 78810-2
Cathepsin K Inhibitor Screening Assay Kit
BPS Bioscience
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DESCRIPTION
The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.
The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.
DETAILS
- Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
- Uniprot: P43235
- Shiptemp: -80°C (dry ice)
- Synonyms: CTSK, CTSO, CTSO2, Cathepsin O, Cathepsin O2, Cathepsin X
- Warnings: Avoid freeze/thaw cycles
- Category: Protease/Assay Kit
- Background: Cathepsin K is a potent protease that belongs to the lysosomal cysteine protease family. It mediates bone resorption and is an attractive target for anti-osteoporosis drug development. The protease has also been associated with skeletal homeostasis as mutation in the gene encoding cathepsin K is linked to a rare autosomal recessive disorder, pycnodysostosis, which is characterized by abnormal bone density and decreased bone turnover. Further, cathepsin K has been implicated in diseases of the central nervous system (CNS), cardiovascular system, and the respiratory system. Studying cathepsin K can lead to a better understanding of its role in disease beyond the musculoskeletal system.
- Description: The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.
- Methodology: Fluorogenic
- Supplied As: The Cathepsin K assay kit comes in a 96-well format, with purified Cathepsin K, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
- Unspsc Code: 41105331
- Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
- Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 79590, 79971, 78589, 78591
- Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
- Scientific Category: Protease