Anti-p21/CIP1/WAF1 Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CDKN1A
Host: Mouse
Rrid: AB_3068400
Type: Primary Antibody
Clone: M513
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: p21/CIP1/WAF1
Isotype: IgG2a
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 MDA-6 p21 CDKN1A CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Clonality: Mouse Monoclonal
Immunogen: Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC), HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluorescent labeling in human cells.
Applications: WB, ICC, IHC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 21
Dilution Range Ip: 1:50
Dilution Range Wb: 1:500
Immunogen Species: Human
Dilution Range Icc: 1:50
Dilution Range Ihc: 1:50
Species Reactivity: Human
Target Description: The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.
Uniprot Number Immunogen Species: P38936