DESCRIPTION

• Tag: C-His
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Source: HEK293 Cells
• Species: Human
• Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) (Cat: 10698-H08H) at 2 μg/ml (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag) (Cat: 11159-H03H). The EC50 of of CTLA-4 (His & hFc Tag) (Cat: 11159-H03H) is 35-80 ng/ml. 2.Loaded Recombinant Human CTLA-4 Protein, hFc Tag (Cat.No.11159-H02H6) on ProA Biosensor, can bind Recombinant Human B7-1 Protein, His Tag (Cat.No.10698-H08H) with an affinity constant of 5.331nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested). 3.Loaded Recombinant Human B7-1 Protein, His Tag(Cat. No. 10698-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 0.46 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested). 4.Loaded Recombinant Human CD86 Protein, His Tag(Cat. No. 10699-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 2.72 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
• Molecule: CD80
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Lead Time: 1-3 days
• Formulation: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human B7-1 consists of 219 amino acids and has a predicted molecular mass of 25.4 kDa. By reduced SDS-PAGE, the apparent molecular mass of rh B7-1 is approximately 45-48 kDa due to glycosylation.
• Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
• Accession Number: NP_005182.1
• Predicted N Terminal: Val 35