DESCRIPTION

• Mw: 78.1 kDa
• Tag: Myc-DDK
• Accn: NM_001199042
• Type: Human Tagged ORF Clone
• Refseq: NM_001199042.2
• Status: 4 Weeks*
• Symbol: STRA6
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 64220
• Orf Size: 2118 bp
• Synonyms: MCOPCB8; MCOPS9; PP14296
• Refseq Orf: 2121 bp
• Uniprot Id: Q9BX79
• Availability: 4 Weeks
• Cytogenetics: 15q24.1
• Gene Summary: The protein encoded by this gene is a membrane protein involved in the metabolism of retinol. The encoded protein acts as a receptor for retinol/retinol binding protein complexes. This protein removes the retinol from the complex and transports it across the cell membrane. Defects in this gene are a cause of syndromic microphthalmia type 9 (MCOPS9). Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC231385 representing NM_001199042 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGGCGGGAAGGGAGGAGGGGACACGAGAGGCCCTGTCCTCTTCCCGTGCCAGCTTGCTCAGGCCCTCA GTCCCAGACGGGCTTTTCCCAGAGAGCTAAAAGAGAAGGGCCAGAGAATGTCGTCCCAGCCAGCAGGGAA CCAGACCTCCCCCGGGGCCACAGAGGACTACTCCTATGGCAGCTGGTACATCGATGAGCCCCAGGGGGGC GAGGAGCTCCAGCCAGAGGGGGAAGTGCCCTCCTGCCACACCAGCATACCACCCGGCCTGTACCACGCCT GCCTGGCCTCGCTGTCAATCCTTGTGCTGCTGCTCCTGGCCATGCTGGTGAGGCGCCGCCAGCTCTGGCC TGACTGTGTGCGTGGCAGGCCCGGCCTGCCCAGCCCTGTGGATTTCTTGGCTGGGGACAGGCCCCGGGCA GTGCCTGCTGCTGTTTTCATGGTCCTCCTGAGCTCCCTGTGTTTGCTGCTCCCCGACGAGGACGCATTGC CCTTCCTGACTCTCGCCTCAGCACCCAGCCAAGATGGGAAAACTGAGGCTCCAAGAGGGGCCTGGAAGAT ACTGGGACTGTTCTATTATGCTGCCCTCTACTACCCTCTGGCTGCCTGTGCCACGGCTGGCCACACAGCT GCACACCTGCTCGGCAGCACGCTGTCCTGGGCCCACCTTGGGGTCCAGGTCTGGCAGAGGGCAGAGTGTC CCCAGGTGCCCAAGATCTACAAGTACTACTCCCTGCTGGCCTCCCTGCCTCTCCTGCTGGGCCTCGGATT CCTGAGCCTTTGGTACCCTGTGCAGCTGGTGAGAAGCTTCAGCCGTAGGACAGGAGCAGGCTCCAAGGGG CTGCAGAGCAGCTACTCTGAGGAATATCTGAGGAACCTCCTTTGCAGGAAGAAGCTGGGAAGCAGCTACC ACACCTCCAAGCATGGCTTCCTGTCCTGGGCCCGCGTCTGCTTGAGACACTGCATCTACACTCCACAGCC AGGATTCCATCTCCCGCTGAAGCTGGTGCTTTCAGCTACACTGACAGGGACGGCCATTTACCAGGTGGCC CTGCTGCTGCTGGTGGGCGTGGTACCCACTATCCAGAAGGTGAGGGCAGGGGTCACCACGGATGTCTCCT ACCTGCTGGCCGGCTTTGGAATCGTGCTCTCCGAGGACAAGCAGGAGGTGGTGGAGCTGGTGAAGCACCA TCTGTGGGCTCTGGAAGTGTGCTACATCTCAGCCTTGGTCTTGTCCTGCTTACTCACCTTCCTGGTCCTG ATGCGCTCACTGGTGACACACAGGACCAACCTTCGAGCTCTGCACCGAGGAGCTGCCCTGGACTTGAGTC CCTTGCATCGGAGTCCCCATCCCTCCCGCCAAGCCATATTCTGTTGGATGAGCTTCAGTGCCTACCAGAC AGCCTTTATCTGCCTTGGGCTCCTGGTGCAGCAGATCATCTTCTTCCTGGGAACCACGGCCCTGGCCTTC CTGGTGCTCATGCCTGTGCTCCATGGCAGGAACCTCCTGCTCTTCCGTTCCCTGGAGTCCTCGTGGCCCT TCTGGCTGACTTTGGCCCTGGCTGTGATCCTGCAGAACATGGCAGCCCATTGGGTCTTCCTGGAGACTCA TGATGGACACCCACAGCTGACCAACCGGCGAGTGCTCTATGCAGCCACCTTTCTTCTCTTCCCCCTCAAT GTGCTGGTGGGTGCCATGGTGGCCACCTGGCGAGTGCTCCTCTCTGCCCTCTACAACGCCATCCACCTTG GCCAGATGGACCTCAGCCTGCTGCCACCGAGAGCCGCCACTCTCGACCCCGGCTACTACACGTACCGAAA CTTCTTGAAGATTGAAGTCAGCCAGTCGCATCCAGCCATGACAGCCTTCTGCTCCCTGCTCCTGCAAGCG CAGAGCCTCCTACCCAGGACCATGGCAGCCCCCCAGGACAGCCTCAGACCAGGGGAGGAAGACGAAGGGA TGCAGCTGCTACAGACAAAGGACTCCATGGCCAAGGGAGCTAGGCCCGGGGCCAGCCGCGGCAGGGCTCG CTGGGGTCTGGCCTACACGCTGCTGCACAACCCAACCCTGCAGGTCTTCCGCAAGACGGCCCTGTTGGGT GCCAATGGTGCCCAGCCC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC231385 representing NM_001199042 Red =Cloning site Green =Tags(s) MGGKGGGDTRGPVLFPCQLAQALSPRRAFPRELKEKGQRMSSQPAGNQTSPGATEDYSYGSWYIDEPQGG EELQPEGEVPSCHTSIPPGLYHACLASLSILVLLLLAMLVRRRQLWPDCVRGRPGLPSPVDFLAGDRPRA VPAAVFMVLLSSLCLLLPDEDALPFLTLASAPSQDGKTEAPRGAWKILGLFYYAALYYPLAACATAGHTA AHLLGSTLSWAHLGVQVWQRAECPQVPKIYKYYSLLASLPLLLGLGFLSLWYPVQLVRSFSRRTGAGSKG LQSSYSEEYLRNLLCRKKLGSSYHTSKHGFLSWARVCLRHCIYTPQPGFHLPLKLVLSATLTGTAIYQVA LLLLVGVVPTIQKVRAGVTTDVSYLLAGFGIVLSEDKQEVVELVKHHLWALEVCYISALVLSCLLTFLVL MRSLVTHRTNLRALHRGAALDLSPLHRSPHPSRQAIFCWMSFSAYQTAFICLGLLVQQIIFFLGTTALAF LVLMPVLHGRNLLLFRSLESSWPFWLTLALAVILQNMAAHWVFLETHDGHPQLTNRRVLYAATFLLFPLN VLVGAMVATWRVLLSALYNAIHLGQMDLSLLPPRAATLDPGYYTYRNFLKIEVSQSHPAMTAFCSLLLQA QSLLPRTMAAPQDSLRPGEEDEGMQLLQTKDSMAKGARPGASRGRARWGLAYTLLHNPTLQVFRKTALLG ANGAQP TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Transmembrane
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin