FcGRIIIA (CD16a) Lentivirus

DESCRIPTION

Fc Gamma Receptor IIIa (FcGRIIIA; FcγRIIIA), also known as CD16a, is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, antibody-dependent cytotoxicity and clearance of immune complexes. The human FcγRIIIa displays a dimorphism in the position of residue 158. One allele (V158) encodes a higher Fc affinity receptor variant with a valine at amino acid residue 158, and the other (F158) encodes a lower Fc affinity receptor variant having a phenylalanine at amino acid residue 158.The FcGRIIIA Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a FcGRIIIA gene (NM_001127593.1; high affinity V158 variant) driven by a CMV promoter.

DETAILS

Shiptemp: -80°C (dry ice)
Synonyms: CD16a Lentivirus
Warnings: Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Category: Cell Line/Lentiviruses
Description: Fc Gamma Receptor IIIa (FcGRIIIA; FcγRIIIA), also known as CD16a, is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, antibody-dependent cytotoxicity and clearance of immune complexes. The human FcγRIIIa displays a dimorphism in the position of residue 158. One allele (V158) encodes a higher Fc affinity receptor variant with a valine at amino acid residue 158, and the other (F158) encodes a lower Fc affinity receptor variant having a phenylalanine at amino acid residue 158.The FcGRIIIA Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a FcGRIIIA gene (NM_001127593.1; high affinity V158 variant) driven by a CMV promoter.
Formulation: The lentiviruses were produced from HEK293T cells in the medium containing 90% DMEM + 10% FBS.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥5 x 106 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: 1. Transient expression of FcGRIIIA in target cells.2. Generation of stable cell line expressing FcGRIIIA with Geneticin (G418) selection.
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 79539, 79564, 79877, 79878
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Scientific Category: Cell Signaling Pathway