mouse monoclonal IgG1; MyoD Antibody (5.8A) is an IgG1 κ mouse monoclonal MyoD antibody that detects MyoD of mouse, rat and human origin by WB, IP, IF and IHC(P). MyoD Antibody (5.8A) is available as both conjugated and non-conjugated anti-MyoD antibody forms. Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either MyoD or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.