Anti-ALDH1A1 (N-terminal region) Antibody

DETAILS

Form: Protein G Purified
Gene: ALDH1A1
Host: Mouse
Rrid: AB_3068361
Type: Primary Antibody
Clone: M562
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: ALDH1A1 (N-terminal region)
Isotype: IgG2a
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: RALDH, ALDH-E1, ALHDII, Aldehyde dehydrogenase, ALDC, PUMB1, ALDH1A1
Clonality: Mouse Monoclonal
Immunogen: Clone M562 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human ALDH1A1.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody antibody detects a 55 kDa* protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 55
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human
Target Description: Aldehyde dehydrogenase (ALDH) superfamily is a ubiquitous group of enzymes found in all taxonomic domains. ALDH detoxifies endogenous and exogenous aldehydes, protecting cellular homeostasis and organismal functions. These enzymes are necessary for the synthesis of retinoic acid, betaine, and folate. Recent studies have reported high levels of ALDH found in cancer cells, suggesting that ALDH can act as a marker for cancer cells found in a wide variety of tissues including skin, prostate, lung, and neural tissues. Additionally, certain diseases can be identified when ALDH activity is absent. ALDH1A1 is vital for retinol synthesis and alcohol metabolism. ALDH1A1 active sites include an active cysteine residue, which catalyses the transformation of aldehydes into their respective carboxylic groups. ALDH1A1 amino acid sequence and function is highly conserved in humans and rodents.
Uniprot Number Immunogen Species: P00352
Post Translational Modification Type: GOmAb