Phospho-SLP-76 (Tyr128) (Clone: 3F8) rabbit mAb APC conjugate

DESCRIPTION

SH2 Domain-Containing Leukocyte Protein Of 76 KDa (SLP-76) is an adaptor protein that plays a role in signal transduction in T cells. Studies using a SLP-76-deficient T cell line have demonstrated that SLP-76 is required for optimal phosphorylation and activation of both PLCg1 and the Ras pathway. SLP-76 phosphorylation is mediated by Zap70 upon TCR stimulation. Within an N-terminal acidic region, SLP-76 possesses three tyrosines (Tyr113, 128, and 145), which are phosphorylated upon activation. The sterile alpha-motif (SAM) domain of SLP-76 drives formation of dimers and higher order oligomers. SLP-76 micro-clusters at the immunological synapse enhance signal transduction and T cell activation.

DETAILS

App Id: FACS
Isotype: Rabbit IgG1k
Format Id: Conjugated
Clone Name: SLP76Y128-3F8
Host Animal: Rabbit
Clonality Id: Monoclonal
Conjugate Id: APC
Reactivity Id: Human, Mouse
Product Content: 1X PBS, 0.09% NaN3, 0.2% BSA
Alternative Name: Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP76, LCP2
Application Note: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Condition: Store at 2-8°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: A synthetic phospho-peptide corresponding to residues surrounding Tyr128 of human phospho SLP-76