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63002-Z08H

Scientist.com Supplier

Danio rerio (zebrafish) EFNB2A / Ephrin B2a Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus.

DETAILS

  • Tag: C-His
  • Purity: > 95 % as determined by SDS-PAGE
  • Source: HEK293 Cells
  • Species: Danio rerio (zebrafish)
  • Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 μg/ml (100 μl/well) can bind human EphB4-Fc (Cat:10235-H02H), The EC50 of human EphB4-Fc (Cat:10235-H02H) is 15.5-36.3 ng/ml.
  • Category: Proteins
  • Molecule: EFNB2A
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, PH 7.4.
  • Molecular Mass: The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 KDa band in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: O73874
  • Predicted N Terminal: Leu 25