71193
TPH2, His-tag (Human) Recombinant
BPS Bioscience
DESCRIPTION
Human tryptophan
hydroxylase 2 (TPH2) also known as
neuronal tryptophan hydroxylase,
GenBank Accession No. NM_173353, a.a.
151-466 (end) with an N-terminal His-tag,
MW = 37 kDa, expressed in an E. coli cell
expression system.
DETAILS
- Aa: 151-466 (end)
- Mw: 37 kDa
- Tags: N-terminal His-tag
- Format: Aqueous buffer solution
- Genbank: NM_173353
- Species: Human
- Uniprot: Q8IWU9
- Shiptemp: -80°C (dry ice)
- Synonyms: neuronal tryptophan hydroxylase, tryptophan hyrdoxylase 2
- Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
- Category: Immunotherapy/Protein
- References: 1. Zill, P., et al., Mol. Psychiatr. 2004; 9: 1030-1036.2. Sheehan, K., et al., Mol. Psychiatr.2005; 10: 944-949.
- Description: Human tryptophan hydroxylase 2 (TPH2) also known as neuronal tryptophan hydroxylase, GenBank Accession No. NM_173353, a.a. 151-466 (end) with an N-terminal His-tag, MW = 37 kDa, expressed in an E. coli cell expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 160 mM imidazole
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Product Type: Protein
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 µM tryptophan, 100 mM ammonium sulfate, 100 µM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH2. Fluorescence signal was measured at Em360nm/Ex300nm.
- Related Products: 71192, 72053, 72054, 72056, 72057
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Metabolic Enzyme, TPH2