Anti-α-Tubulin (C-terminus) Antibody

DETAILS

Form: Protein A Purified
Gene: TUBA1
Host: Mouse
Rrid: AB_10703566
Type: Primary Antibody
Clone: DM1A
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: α-Tubulin (C-terminus)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: alpha Tubulin, Tubulin A1C
Clonality: Mouse Monoclonal
Immunogen: Clone DM1A was generated from purified chick brain tubulin. The antibody recognizes an epitope within the C-terminal end of α-tubulin isoforms. The epitope is highly conserved in α-tubulin isoforms from most vertebrate species.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 55 kDa* protein corresponding to the molecular mass of α-Tubulin on SDS-PAGE immunoblots of human, rat, and mouse cells and tissues.
Applications: WB, ICC, IHC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 55
Dilution Range Wb: 1:500
Immunogen Species: Chicken
Dilution Range Icc: 1:200
Species Reactivity: Human, Mouse, and Rat
Target Description: Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.
Uniprot Number Immunogen Species: P02552