CP3081
Anti-CrkL (C-terminus) Antibody
PhosphoSolutions
DETAILS
- Form: Antigen Affinity Purified
- Gene: CRKL
- Host: Rabbit
- Rrid: AB_3068407
- Type: Primary Antibody
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: CrkL (C-terminus)
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: v-crk sarcoma virus CT10 oncogene homolog, CRKII, CRKL
- Clonality: Rabbit Polyclonal
- Immunogen: CrkL synthetic peptide (coupled to KLH) containing amino acid in the C-terminus of human CrkL. This peptide sequence is well conserved in mouse and rat CrkL, and has less than 50% homology to human Crk II.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: The antibody detects a 38 kDa* protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells, mouse brain tissue, and NGF-differentiated rat PC12 cells.
- Applications: WB
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 38
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Species Reactivity: Chicken, Human, Mouse, Rat, and Xenopus
- Target Description: The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.
- Uniprot Number Immunogen Species: P46109