DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Format: aqueous solution containing DMSO
• Uniprot: Q16236
• Shiptemp: -80°C (dry ice)
• Synonyms: ARE cell line, Nrf2 reporter, Nrf
• Warnings: Avoid freeze/thaw cycles.
• Category: Cell Signaling/Cell Line
• Background: The Nrf2 antioxidant response pathway plays an important role in cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.
• References: 1. Lee, JM et al. (2004) An important role of Nrf2-ARE pathway in the cellular defense mechanism. J Biochem Mol Biol. 37(2): 139-143.2. Dinkova-Kostova, AT et al. (2002) Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants. Proc Natl Acad Sci U S A. 99(18): 11908-11913.
• Description: The ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.
• Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: • Monitor Nrf2 antioxidant response pathway activity.• Screen for activators or inhibitors of the Nrf2 antioxidant response pathway.
• Host Species: human
• Product Type: Cell Line
• Host Cell Line: HepG2
• Biosafety Level: BSL-1
• Related Products: 60514, 79533, 60690, 60187, 79796
• Storage Stability: Store in liquid nitrogen immediately upon receipt.
• Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
• Biological Activity: This cell line is validated for the response to the stimulation of tert-butylhydroquinone and sulforaphane.
• Scientific Category: Antioxidant Signaling pathway
• Instructions for Use: See data sheet for detailed culturing and assay protocol.
• Required Accessories: 79533,60690,60187,79796
• Signed License Required: Yes